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How To Sign Up For Clinical Trials For Skin Rejuvenation

  • Periodical Listing
  • Photomed Laser Surg
  • PMC3926176

Photomed Laser Surg. 2014 Feb ane; 32(2): 93–100.

A Controlled Trial to Determine the Efficacy of Red and Nigh-Infrared Light Treatment in Patient Satisfaction, Reduction of Fine Lines, Wrinkles, Peel Roughness, and Intradermal Collagen Density Increment

Alexander Wunsch

1Medical Light Consulting, Heidelberg, Frg.

Karsten Matuschka

iiJK-International GmbH, Windhagen, Frg.

Abstract

Objective: The purpose of this study was to investigate the safety and efficacy of two novel lite sources for large surface area and full body application, providing polychromatic, non-thermal photobiomodulation (PBM) for improving skin feeling and advent. Background information: For non-thermal photorejuvenation, laser and LED light sources accept been demonstrated to exist safe and effective. However, lasers and LEDs may offer some disadvantages because of dot-shaped (punctiform) emission characteristics and their narrow spectral bandwidths. Considering the action spectra for tissue regeneration and repair consist of more than one wavelength, we investigated if it is favorable to employ a polychromatic spectrum covering a broader spectral region for skin rejuvenation and repair. Materials and methods: A total of 136 volunteers participated in this prospective, randomized, and controlled written report. Of these volunteers, 113 subjects randomly assigned into four treatment groups were treated twice a week with either 611–650 or 570–850 nm polychromatic lite (normalized to ∼ix J/cm2 in the range of 611–650 nm) and were compared with controls (due north=23). Irradiances and treatment durations varied in all treatment groups. The data collected at baseline and later on xxx sessions included blinded evaluations of clinical photography, ultrasonographic collagen density measurements, computerized digital profilometry, and an assessment of patient satisfaction. Results: The treated subjects experienced significantly improved skin complexion and pare feeling, profilometrically assessed skin roughness, and ultrasonographically measured collagen density. The blinded clinical evaluation of photographs confirmed meaning improvement in the intervention groups compared with the command. Conclusions: Broadband polychromatic PBM showed no advantage over the red-light-merely spectrum. However, both novel calorie-free sources that have not been previously used for PBM take demonstrated efficacy and prophylactic for peel rejuvenation and intradermal collagen increase when compared with controls.

Introduction

Altering cellular function using depression level, non-thermal LED light is called photobiomodulation (PBM) or low-level calorie-free therapy (LLLT), and is a medical treatment modality of increasing clinical importance.1 Because of the combination of high degree of penetration in pare2 and absorption by respiratory chain components, light in the spectral range from 600 to 1300 nm is useful for promoting wound healing, tissue repair, and skin rejuvenation.three–5 In contrast to traumatic ablative (e.yard., laser resurfacing) and non-ablative (e.g., intense pulsed lite [IPL]) peel rejuvenation modalities that induce secondary tissue repair by causing controlled harm to either the epidermis or the dermis, PBM is atraumatic, and bypasses the initial destructive step by directly stimulating regenerative processes in the skin. Its action mechanisms encompass increased cellular proliferation, migration, and adhesion.half-dozen Of import cell types for skin and tissue regeneration are fibroblasts, keratinocytes, and allowed cells (mast cells, neutrophils, and macrophages), which can be stimulated using specific wavelengths with significant tissue penetration properties.7 The known astringent side effects of traumatic skin rejuvenation procedures, such equally inflammation, unpleasant hurting perception, and prolonged social downwardly time,eight are unknown in PBM; PBM has been successfully administered to reduce common symptoms of laser resurfacing and IPL treatment.9 Photon emitters, such as lasers or LEDs, have proven to be effective light sources for PBM during recent decades, thereby demonstrating that information technology is not the technical type of light source but the handling parameters such as wavelength, irradiance, and fluence that are probable to be answerable for the effects.10 However, light amplification by stimulated emission of radiation and LED low-cal sources may offer some disadvantages because of their dot-shaped (punctiform) emission characteristics and narrow spectral bandwidths. Considering the activity spectra for tissue regeneration and repair consist of more than than 1 wavelength,seven,eleven information technology might exist favorable to apply a polychromatic spectrum covering a broader spectral region for skin rejuvenation and skin repair. We investigated the safety and efficacy of a novel non-thermal, non-ablative, atraumatic, polychromatic low-level light handling modality with a focus on pleasant skin feeling, improved skin advent, intradermal collagen increase, and the visible reduction of fine lines and wrinkles in a prospective, randomized, controlled trial that consisted of 136 volunteers.

Materials and Methods

Written report population and pattern

We conducted a randomized, controlled clinical trial between Jan 2012 and December 2012. Table 1 summarizes the baseline (t0) characteristics of the subject groups.

Table 1.

Baseline (t0) Characteristics of the Subject Groups

RLT (n=57) ELT (north=48) Controls (north=23)
Sex
 Female 49/86.0% 34/70.8% 15/65.2%
 Male person 8/14.0% 14/29.2% 8/34.8%
Age a 46.2±9.0 48.6±ix.8 44.4±10.2
Weight a 72.9±xv.22 73.4±13.vii 73.7±13.four
Skin complexion (subjective) b 4.54±1.92 4.87±two.02
Peel feeling (subjective) b 5.33±2.04 5.24±2.18
Skin roughness (Ra) b 15.29±four.xx xiv.84±4.04 11.79±2.17
Collagen intensity score c xx.twoscore±six.55 18.96±3.54 23.22±7.36
Expert contraction assessment d
 No/shallow or fine wrinkles 14/24.half-dozen% 17/35.4% 5/21.7%
 Moderate wrinkles 20/35.i% xi/22.9% 6/26.1%
 Prominent or deep wrinkles xiii/22.8% 11/22.9% 9/39.1%
 No majority vote possible 10/17.v% 9/18.8% iii/13.0%

The subjects were betwixt 27 and 79 years of age. Inclusion criteria were the capacity to independently position oneself to utilise the device, the capacity to understand the handling, a signed proclamation of consent, and involvement in continuous participation. The exclusion criteria were physical and psychological disease casting doubt on the capacity to consent, preliminary treatment with reddish light within the 6 months prior to the beginning of the report, recent invasive cosmetic procedures such every bit Botox during the 12 months prior to the beginning of the study, astute or prior skin cancer, acute skin affliction requiring dermatological treatment, existing or planned pregnancy, lactation, history of photosensitivity or recent use of photosensitizing medication, epilepsy, and the tendency to faint. All of the participants gave written informed consent for this report, which was approved by the Ethics Committee of the Medical Association (Landesärztekammer) Baden-Württemberg, Stuttgart, Deutschland. The investigation was conducted in accordance with the Declaration of Helsinki (DoH/Oct2008). Subsequently the declaration of informed consent following examination of the inclusion and exclusion criteria, each participant was assigned to one of four groups using a computerized randomization procedure. Group v was mainly recruited from employees of the JK company without randomization, and served as the command. Groups 1–4 were treated twice a week with 30 treatments in total, starting in Jan 2012. To minimize the influence of seasonal changes, the time interval for data acquisition at the baseline, t15, t30, and follow-upward examinations was restricted to 1 month. The data conquering at baseline was completed in February 2012, and all of the volunteers finished treatment 30 (t30) in June 2012.

The control group did not receive whatsoever handling, as the therapy cannot be blinded, and a sham light source without any event nearly likely does not exist. The command group volunteers participated in the clinical measurements only, and the acquisition of subjective parameters such as skin feeling and peel complexion was not conducted. Considering of the like spectral lamp characteristics for groups 1 and ii and groups three and iv, groups 1 and two were combined for evaluation as the "mid-pressure lamp group" [energizing calorie-free technology (ELT)], and groups iii and 4 were evaluated together as the "low-pressure lamp group" [crimson light engineering science (RLT)] to obtain larger group sizes and, therefore, higher statistical power. Withal, the subdivision into groups 1–4 immune us to compare outcomes based on dissimilar treatment parameters, such as spectral distribution, irradiance, and fluence. A questionnaire apropos the tolerability of the application was filled in after each handling (t1–t30). Digital photographs and clinical measurements were taken, and subjective questionnaires were used to assess complexion and skin feeling at the baseline (t0) and after fifteen (t15) and 30 treatments (t30). The follow-upwardly conquering of subjective and clinical parameters was conducted at t30+6 months.

Lite Sources

Four units equipped with 2 different types of polychromatic light sources (low-pressure vs. mid-pressure level lamps) were used to deport this study. Table 2 lists the lamp technologies, lamp types, treatment area (total or office of the torso), spectral values, session elapsing, and handling doses for the units used in this study.

Tabular array ii.

Characteristics of the Handling Units, Calorie-free Sources, and Awarding Parameters

Treatment units (groups ane – iv)
ELT two ELT 30 C 46 sun CVT/RVT
Technology Energizing light (ELT) Energizing light (ELT) Red light (RLT) Red light (RLT)
Lamp type Medium pressure Medium pressure Depression pressure Low pressure
Handling expanse Fractional-torso Total-body Total-torso Total-torso
Treatment position Semi-reclined Horizontal Horizontal Vertical
Irradiance (611–650 nm) seven.1 mW/cm2 10.4 mW/cm2 5.9 mW/cmii xiii.3 mW/cmii
Total irradiance (570–850 nm) 42.8 mW/cm2 54.viii mW/cmii x.3 mW/cm2 23.iv mW/cm2
Treatment duration 20 min xv min 25 min 12 min
Treatment dose (611–650 nm) 8.5 J/cm2 9.iv J/cm2 8.9 J/cmtwo 9.6 J/cm2
Total radiant exposure (570–850 nm) 51.4 J/cm2 49.3 J/cm2 15.5 J/cm2 xvi.8 J/cmtwo

Treatment units 2, 3, and 4 provided full-body irradiation, covering the ventral and dorsal surfaces of the head, cervix, trunk, upper limbs, and lower limbs at the same time. Full-torso irradiation units ii and iii enabled treatment with the patient in a horizontal, reclined position, whereas unit 4 was engineered as a cabin for vertical treatment orientation. Unit ane was designed for the local treatment of the face and décolletage expanse with the patient sitting in a chair in a semi-reclined position. Units 1 and 2 were equipped with medium-pressure gas discharge lamps in combination with spectrally selective reflectors and corresponding filter systems, to eliminate spectral emissions in wavelengths <570 and >850 nm; these units were denoted as ELT. Units three and iv were equipped with low-pressure gas belch fluorescent lamp tubes providing a spectral emission superlative predominantly inside the range of 611–650 nm, denoted every bit RLT. Because of the unlike spectral properties and irradiances, we defined the spectral range between 611 and 650 nm for the calculation of treatment fluences. This wavelength window encompasses 632.8 nm, which is a paramount wavelength in LLLT and PBM, representing the ascendant wavelength of a HeNe-laser. The spectral dose distributions of the ELT and RLT low-cal sources are shown in Fig. 1, with the doses of both light sources normalized to 100 % for the 611–650 nm range. The treatment doses were kept abiding for this spectral range, whereas irradiances and treatment durations varied for all four treatment groups in society to investigate the applicability of the Bunsen–Roscoe law of reciprocity within the given parametrical limits.

An external file that holds a picture, illustration, etc.  Object name is fig-1.jpg

Spectral dose distributions of energizing light engineering (ELT) and red light technology (RLT) calorie-free sources. Relationship between doses and wavelength ranges for ELT and RLT light sources, normalized to the spectral range 611–650 nm. Colored bars represent the spectral doses in percentages.

All units emitted almost no erythemogenic UV radiation (minimal erythema dose would not be reached after several hours of exposure, comparable to the UV emission of fluorescent lamps for general lighting service applications).

Measurements

The principal objective of the written report was the improvement of subjective pare complexion and skin feeling. The volunteers were asked to specify their level of agreement to the statements in the questionnaire by marking a position along a continuous blackness line betwixt 2 cease points measuring 10 cm, which served equally a visual analog scale (VAS). The secondary objectives were the comeback of measurement parameters using a DermaLab Combo (Cortex Technology, Hadsund, Denmark), a reckoner-supported skin diagnostics system equipped with a rotating high-resolution ultrasound sensor probe (20 MHz) for the decision of changes in intradermal collagen density, measured as a collagen intensity score (CIS). A Primoscalorie-free digital fringe projection arrangement (GFM Messtechnik, Berlin, Germany) was used to measure out the objective arithmetical roughness (Ra) of the skin surface in the periorbital region.

Photography

The digital photographs for the blinded contraction assessment were taken using a Nikon D5100 camera equipped with a Nikkor AF 50 mm 1:1.four lens (Nikon Corporation, Chiyoda, Tokyo, Japan) and a Walimex RFL-3 ring light (Walser GmbH & Co. KG, Burgheim, Germany).

Subject effect assessment

The subjective efficacy parameters were self-assessed at the baseline (t0), after fifteen (t15) and 30 (t30) treatments, and subsequently t30+6 months using 10 cm VAS for the improvements in skin complexion and skin feeling. These parameters were non assessed in the control group.

Objective clinical parameter assessment

The high-resolution ultrasound exam of collagen has enabled the measurement of visible changes in collagen density and numerical CISs representing the intradermal collagen fiber density. Profilometry yielded a numerical value for the Ra of the skin expanse nether examination.

Investigator assessment

Three independent physicians who were blinded to the clinical patient data, analyzed the clinical photographs obtained at t0 and t30. The investigators were instructed to arrange the randomly contrasted sets of clinical photographs taken at t0 and t30 into a before/after treatment sequence. The baseline wrinkle depth according to the Modified Fitzpatrick Wrinkle Calibration (MFWS)12 and the caste of wrinkle reduction afterward handling had to be assessed after sequencing. The votes of the investigators were summarized by the following majority rules: if two or three experts voted the same mode, the agreed-upon nomenclature was the summary measure; if all 3 experts voted differently, "no alter" was the summary measure.

Statistical methods

The information in the tables are given equally means±standard deviations. Comparisons of the changes in skin feeling, skin complexion, roughness, and collagen intensity from the baseline to t30 between the different treatment groups (intergroup comparisons) were performed using a linear model, with the baseline value of each volunteer as a covariate. Within-group differences from the baseline to values at t30 were assessed using the Isle of mann–Whitney–Wilcoxon test. To compare wrinkle difference assessments among groups, nosotros used the χ2 examination. Inside groups, nosotros tested the hypothesis of equal probabilities of improvement and worsening using binomial tests. All tests were two sided, and p values<0.05 were considered statistically significant.

Results

Patient characteristics

Initially, 144 volunteers were recruited for the trial. Eight volunteers did non appear for the starting time appointment after randomization; therefore, the full number of patients finally included in the study was 136. Five volunteers stopped participating because of schedule incompatibilities and lack of time. One volunteer could not finish the treatment because of receiving antibiotic medication, which was 1 of the exclusion criteria; one volunteer terminated participation because of moving away; and one participant missed more than than 4 treatments because of a period of residence at a health resort. Ultimately, 128 volunteers completed the handling and the follow-up evaluation course, of whom 57 were treated with RLT, 48 were treated with ELT, and 23 were controls. The volunteers in the RLT and ELT groups were similar with respect to age, weight, skin complexion, peel feeling, pare roughness, and intradermal collagen density. The percentage of women was lower in the ELT group than in the RLT group. The controls had a slightly higher mean collagen density and a lower mean skin roughness.

Adverse events

None of the volunteers dropped out because of an adverse outcome. No severe agin events were registered during the study or the follow-up phase. 1 volunteer with facial telangiectasia noticed an increased visibility after the first treatments, and decided to protect the zones in question from the light influence using a concealer for the rest of the treatment serial. One volunteer experienced a reddening of scar tissue from a 40-year-quondam human knee injury that was probable reactivated past the ELT thirty treatment. The afflicted scar healed completely within 1 week, and the treatments were continued without intermission.

Assessment of effects

Figure 2 shows two serial of collagen ultrasonography scans, demonstrating the collagen density increase from t0 to t30 for one subject each in the RLT group and the ELT group.

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Collagen ultrasonography examples.

Clinical photography revealed visible changes in wrinkles and skin roughness. Figure 3 shows an example for one bailiwick in each treatment group, comparing the baseline (t0) status with t30.

An external file that holds a picture, illustration, etc.  Object name is fig-3.jpg

Patient photography examples. (A) 64-yr-sometime woman, energizing lite technology (ELT). (B) 41-year-old woman, cherry-red light technology (RLT).

In Table 3, the results of the t30−t0 measurements for each parameter in the different patient groups and the results of the skillful wrinkle cess are summarized. Within-group comparisons addressed whether the t30−t0 differences had means of cipher for each patient group separately.

Table 3.

Comparison of the t30 − t0 Results Between and Inside Discipline Groups

RLT (northward=57) Within-group p value ELT (n=48) Within-grouping p value Controls (n=23) Inside-group p value Between- group p value
Skin complexion (subjective) a −one.29±1.98 <0.001 −1.72±2.35 <0.001 0.064
Skin feeling (subjective) a −1.01±two.30 <0.001 −1.65±two.17 <0.001 0.167
Skin roughness (Ra) a −1.79±2.46 <0.001 −1.58±2.22 <0.001 0.95±1.45 0.003 0.003
Collagen intensity score b 5.75±4.54 <0.001 6.40±five.17 <0.001 −0.26±5.09 0.84 <0.001
Expert wrinkle assessment c <0.001 <0.001 <0.001 <0.001
 Better 40/69% 36/75% ane/4%
 Equal eight/14% 7/15% 5/22%
 Worse 10/17% v/x% 17/74%

Within-group comparisons, t30−t0

In the RLT and ELT groups, skin complexion, skin feeling, collagen intensity score, skin roughness, and wrinkle status improved significantly (p<0.001, Table iii). The skin feeling, skin complexion, and roughness changes were significantly (p<0.001, covariance analysis) correlated with baseline values in all groups. In contrast, the control subjects showed no significant difference in collagen density and significant worsening of peel roughness and contraction status. These results are described in greater detail in Fig. 4. Here, baseline measurements on the x-axis and the respective gain or reduction in the t30 values on the y-centrality are colour coded for the different treatment groups. In Fig. 4A, B and D, virtually all of the ELT and RLT points plotted beneath the baseline x-axis=0.00, indicating that the skin feeling, skin complexion, and roughness improved for most all of the volunteers (p<0.01). In Fig. 4C (CIS), the baseline outcome is not meaning, whereas the CIS increase is significant (p<0.001), and values in a higher place the 10-centrality indicate improvement.

An external file that holds a picture, illustration, etc.  Object name is fig-4.jpg

Results for t30−t0. Changes t30−t0 (y-axis) are depicted in relation to the baseline value t0 on the x-axis. For A, B, and D, points below the x-axis betoken improvement; for C, points above the x-axis indicate improvement. The red light engineering (RLT) and energizing light technology (ELT) t30 − t0 differences decrease with increasing baseline values.

Between-group comparisons

For the main efficacy parameters, skin complexion and skin feeling, we observed no significant differences between the RLT and ELT groups. The collagen density, roughness, and wrinkle status were significantly different amidst the three groups, as shown in Tabular array 3. There was no difference between the RLT and ELT groups, simply there was a difference between both groups compared with controls, as shown by the blue points in Fig. 4C and D.

Subgroup analyses

We wanted to assess whether the ii RLT handling groups and the 2 ELT handling groups showed unlike results; therefore, we compared the ii groups. The RLT subgroups had 25 volunteers using CVT/RVT and 32 using C46 sun. There were no differences between the ii groups with respect to skin complexion, skin feeling, skin roughness, collagen density, and wrinkle condition. All of these parameters improved significantly between t0 and t30 (data not shown). We obtained very similar results for the ii ELT groups, with 27 volunteers in ELT xxx and 21 volunteers in ELT two.

The RLT grouping consisted of a lower per centum of male volunteers than did the ELT grouping and the command. Gender differences regarding the response to the PBM treatment for the main parameters were tested within each of the RLT/ELT/control subgroups using the Isle of mann–Whitney U test, and we found no significant differences (p>0.1 for all tests). Including gender every bit an additional covariate in the covariance assay resulted in very like p values for the tests regarding the comparison of written report groups, compared with the analysis without gender. Only for collagen increase were gender and treatment both pregnant.

Long-term follow-up

The long-term results were analyzed for all subjects who were available for long-term follow-up in November/December 2012. A total of 52 of the 77 subjects who took part in the long-term follow-upward finished after 30 treatments, 18 volunteers continued to a total of 45 treatments, and 7 volunteers received a total of sixty treatments (t60). To analyze the long-term furnishings, nosotros tested whether the t60 measurements of peel feeling, pare complexion, CIS, and Ra were better than the t0 measurements for the group of volunteers with xxx treatments. All volunteers had significantly better results at t60 (Wilcoxon test ≤0.001 for each). The t60−t0 differences were equally follows: mean 0.99, SD 1.95 for skin feeling; mean −1.00, SD two.10 for skin complexion; hateful 5.10, SD 7.56 for CIS; and mean −0.64, SD three.53 for Ra. As expected, these differences displayed lower effect sizes than at t30. Merely a group of 7 volunteers connected the therapy with good results for a further 30 treatments, which may be partly the result of option bias. Therefore, the long-term efficacy must be systematically evaluated in further studies. During the follow-up menstruation, no delayed adverse events were recorded.

Discussion

The utilize of LED light sources with 590, 633, and 830 nm wavelengths for athermal light-only photorejuvenation has grown rapidly in recent years. Additional wavelengths have been shown to exist efficient in altering cellular functions, such as 570,13 620, 680, 760, and 820 nm.14 The treatment doses vary significantly, ranging from 0.1 J/cmtwo for 590 nm LED light with a specific sequence of pulsing,xv up to 126 J/cmtwo for 633 nm continuous LED light.16,17 The power of the low-cal typically ranges betwixt one and grand mW, depending upon the type of light source and the awarding.1 Efficacy comparisons of the different devices available to the doctor are not known to the authors.

This study is the first prospective clinical trial investigating the safety and efficacy of novel light sources for peel rejuvenation and the stimulation of dermal collagen synthesis based on low-pressure level and mid-pressure level gas discharge lamps. These calorie-free sources, in dissimilarity to lasers and LEDs, allow simultaneous treatment with a tailored spectrum composed of several spectral bands that are effective in PBM. When compared with the initial values and the controls, the volunteers experienced significant improvements in their personal assessments of pare feeling and complexion, in clinical outcomes as assessed past collagen density and peel roughness measurements and in the reduction of fine lines and wrinkles every bit assessed by three blinded evaluators comparing t0 and t30 photographs.

Previous findings were able to correlate fibroblast activity and dermal matrix remodeling processes, with an increase in intradermal collagen density and reduced signs of aging.18 The proposed underlying mechanisms include the photostimulation of terminal molecules in the electron ship chain and the subsequent adenosine triphosphate (ATP) concentration increment,xiv along with the selective low-cal-driven activation of water molecules,xix thereby enhancing metabolic substitution and influencing the ion transporter systems found in cellular membranes.xx Detailed analysis of the cistron expression profiles in man fibroblasts revealed an influence of low-intensity red light with a 628-nm wavelength on 111 different genes that are involved in cellular functions, such every bit cell proliferation; apoptosis; stress response; protein, lipid and sugar metabolism; mitochondrial energy metabolism; Deoxyribonucleic acid synthesis and repair; antioxidant related functions; and cytoskeleton- and cell-jail cell interaction-related functions.21 A specific function of reactive oxygen species (ROS) in increasing fibroblast proliferation and movement has recently been reported, suggesting that the height of ROS via photodynamic therapy can enhance the cellular functions of dermal fibroblasts through specific mitogen-activated poly peptide kinase (MAPK) signaling pathways in vitro.22 The lite-induced free radical formation in human skin has been investigated in detail, demonstrating that red light with 620 and 670 nm wavelengths increases the concentration of ROS even without the influence of external photosensitizers.23

Because fibroblasts are responsible for collagen product in wound healing, dermal remodeling, and tissue repair, we decided to focus on increased collagen density as a surrogate marker for fibroblast activeness, and abased such invasive monitoring methods equally histologic examinations post-obit skin biopsies for our report. Ultrasonographic collagen assessment is described as a feasible noninvasive methodology for monitoring dermal density during the senescence procedure.24

A report of the stimulatory furnishings of 660 nm wavelength laser light on scar fibroblasts25 could conceivably explicate the potential reactivation of a>40-yr-erstwhile genu injury, which occurred in i volunteer during the ELT treatment. Therefore, the influence of PBM on scar tissue should exist subject to further investigation.

Some authors emphasize the importance of distinct wavelengths for optimal results.16–18,26–28 In our written report, the differences between the RLT and ELT treatments in clinical outcome and patient satisfaction were not meaning, indicating that despite spectral differences, both light sources were commensurably effective regarding study objectives. Farther studies of the treatment parameters are necessary.

The evaluation of clinical photography revealed a particular worsening of fine lines and wrinkles from t0 to t30 in the control group, which was not expected for a class of merely 12 weeks. A possible explanation could exist the seasonal variation of skin condition between winter and summer climates and the influence of solar radiations, as the clinical photography revealed peel pigmentation as a event of exposure to sunlight.

We observed a tendency that ELT/RLT treatment led to meliorate results in female volunteers regarding the collagen density increase. This gender-specific response could conceivably exist explained by physiological differences between male person and female peel29,30 on endocrine and extracellular matrix levels. Even so, gender-specific differences should be evaluated in greater item in farther investigations.

Conclusions

RLT and ELT are big-expanse and total-body treatment modalities for peel rejuvenation and improvements in skin feeling and peel complexion. The application of RLT and ELT provides a prophylactic, non-ablative, non-thermal, atraumatic photobiomodulation handling of peel tissue with high patient satisfaction rates. RLT and ELT can extend the spectrum of anti-crumbling treatment options available to patients looking for mild and pleasant light-only skin rejuvenation.

Acknowledgments

Nosotros thank Dr. Christine Fischer, Heidelberg, for help and communication regarding the statistical assay of our data. We also thank all of the volunteers for their participation in this study. This study was fully funded by JK-Holding GmbH, Windhagen, Germany. All materials, low-cal sources, and evaluation equipment were provided by the sponsor.

Author Disclosure Argument

The principal investigator (Alexander Wunsch) was mandated and remunerated by the sponsor to deport the study. The authors take received funds to program, comport, and evaluate the study.

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Source: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926176/

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